All FAQs

Magnification vs. Working Distance

Most Dino-Lite microscopes have variable magnification. As with traditional microscopes, a higher magnification means shorter working distance, and smaller field of view and depth of field, and a lower magnification will give a longer working distance, larger field of view and depth of field.

Magnification vs. Working Distance Charts

Focus and Field of View with MS35B »

Download Magnification vs. Working Distance chart PDF »

Field of View

Depth of Field

As the magnification increases, the depth of field decreases.
Download chart PDF »

Field of View

Field of View Demo »

 <
Comparison »

Depth of Field

Download AM413TL vs AM413T Depth of Field Chart chart PDF »

Eyepiece Camera

Eyepiece camera field of view comparison »

Models up to 1.3MP

OS: Windows XP, Vista, 7
(MAC: OSX 10.4 +)
CPU: 1.5GHz Pentium IV or equivalent AMD Athlon processor and higher
HD: At least 200MB available HD space
RAM: 1GB RAM (1.5 require for Windows Vista,7) and above
Graphics card: 32MB video card or above
CD-ROM driver for installation
USB 2.0

Models up to 5MP

OS: Windows XP, Vista, 7
CPU: 2.4 GHz Pentium IV or equivalent AMD Athlon processor or higher
HD: 2GB of available HD space
RAM: 1GB of available RAM
Graphics Card: 64MB video card or above
CD-ROM drive for installation
USB2.0

Feature Comparison Chart »
Software Features Gallery »

User Guide »
Adjust White Balance & Auto Exposure»
Full Screen »
Measurement »
Measurement Calibration »
MicroTouch »
LED Toggle On/Off or Different Type (if applicable) »
Multiple Windows »
Image Settings »
Save Image »
Save Folders »
Annotate & Customize »
Change Unit of Measurement »
Crosshair »
Custom Frames »
Edge Detection »
IP Camera »
Barcode Reader »
Mac OS Software »
Mac OS Measurement »
Mac OS Calibration »

Image Save Format

Windows: BMP, GIF, PNG, MNG, TIF, TGA, PCX, WBMP, JP2, JPC, JPG, PGX, RAS, PNM
Mac: PNG, JPEG

*DinoCapture saves images by default as a bitmap(bmp) file, but this option can be changed.

Video Save Format

Windows: WMV
Mac: MOV

Feature Comparison Chart »
Hardware Features Gallery »

Handheld »
Eyepiece »
Variable Magnification »
Resolution up to 5MP »
Varied Working Distance Options »
Many Lighting Options »
Polarized Lighting Options »
MicroTouch »
Premium Quality Housing »
Connectivity Options »
Hundreds of Accessory Combinations »
Knowledgeable Support Team »
Comprehensive Warranty »

Accessories Demo Images »
All BigC Accessories »

All LED FAQs »

• Award winning product and design
• #1 Selling Handheld Microscope in the World
• Unique design with a single lens with variable magnifications
• Developed for professional use in professional setting, yet affordable for casual or educational usage
• 30+ Models to choose from
• Wide selection of accessories
• User friendly, advanced Software included
• Software can be installed on multiple computers at no additional cost
• Free software updates
• Mac Compatible
• Measurement and calibration feature available
• Applications engineer available
• USA Patent
• Dino-Lite series microscopes are the original patent design for handheld microscope, beware of imitators
• 2 Year warranty on Dino-Lite microscopes and Dino-Eye eyepiece cameras
• 1 Year warranty on BigC Accessories
• Toll Free Technical Support
• 99% Product in stock

There are advantages and disadvantages to both types. The handheld digital microscope with the built in camera is more portable and versatile and can provide up to a 360 degree view perspective but is usually more expensive. Digital camera eyepieces for microscopes are generally less expensive and can be adapted for use as an accessory with any standard microscope. Both types generally come with software which can be easily upgraded via download. Both are self contained units and require only simple cleaning for maintenance. Any serious repair work needs to be handled through the distributor or dealer.

If you require portability, hand-held or mounted versatility and convenience then choose one of the Dino-Lite series of digital microscopes to satisfy these requirements. If you have an existing standard type (biological, stereo or compound) microscope and you want to convert it to a digital microscope, then you should chose a suitable “AM” series digital camera for your microscope.

For slides, a traditional microscope would probably work better, however it would be possible to use handheld.

The Handheld microscopes offer a range of magnification of 10x-50x and also focus again at 200x. However to reach the focal points of 50x and 200x the microscope itself must be touching the object. The handheld microscope is for use for versatile movement and not particularly for slides. The 8 LED lights emanate from within the microscope downward as opposed to traditional microscopes.

• Industrial Inspection
• Assembly and Quality Control (Electronics, Mechanical, etc)
• Scientific teaching tool
• Laboratory Research
• Medical analysis
• School Research tool
• Insect or Plant Dissection/Examination
• Dermatology (Skin/Scalp) Examination
• Textile Inspection
• Gemstone/Mineral Inspection
• Hobbies & Collecting (coin, stamp, watch, jewelry, etc.)
• Printing Inspection
• Law Enforcement (Counterfeit ID, Crime Lab, etc)
• Reading Aid
• On-line chatting
• Teacher Aid as Projector
• Presentation

And many more!

AM4012N

AM4012NZT

AD4012NTL, AD4012MNTL

AD4012MNT

Part A.

1. Place the illuminated specimen on the center of the microscope stage.
2. Always start observing using the lowest magnification with the microscope.
3. Aim the specimen through the objective lens.
4. Focus the microscope by slowly turning the coarse Adjustment Focus Knob, for different magnifications, until an image forms ,slowly adjust the focus back and forth to get the best focus, or to reveal hidden structure at different depths of the specimen being examined.
5. To increase magnification, rotate the magnifications or the Objective Lens depending on the models of the Microscope
6. Focusing becomes more critical when using higher magnifications as the objective lens would need to come closer to the specimen to obtain a focus.
7. Use the same techniques cautions in coarse and fine focusing as described above and use care so that the Objective Lens does not come in direct contact with the specimen when achieving a final focus.
8. CLick on “picture” button or “video” button from DinoCapture software for taking the picture you want or recording the video.
9. What are the applications for Dino digital microscopes? Industrial Inspection, Scientific, teaching tool, Skin Check, Scalp Check, Textile Inspection, Jewelry Inspection, Coin Inspection, Printing Inspection, Reading Aid, Web cam for Online chatting, and more.

Part B.

How would we know what magnification to choose for our imaging products?
Always start observing using the lowest magnification with the microscope.
Adjust the objective lens or magnification knob until the desired image comes into focus.
Then increase magnification gradually until satisfied.

To reduce glare simply change the point of view the angle of either the object or the scope by a few degrees.
On models AM311S and higher you are also able to turn off the LED lights if desired.

The TV Video models utilize NTSC analog format which provides “no-delay” images, whereas the USB models use a digital format which may cause a slight delay when focusing.

The current DinoCapture measurement functions have been tested by major LCD manufacturers to be
within a 5% margin of error under 200 x magnifications.

When examining LCD panels it may be necessary to apply a thin coat of oil (baby oil, etc) to the panel
surface in order to view sub-pixels. The “hard coating” (HC) applied to the LCD panel at the factory
may make this necessary in order to view the pixels beyond the polarize level.

Use time-lapsed capture function problem under Windows OS-
Cannot start after using time-lapsed capture function. only shows dino-lite logo and hangs there.
Possible cause: dino-lite accidentally created a corrupted .avi file from time-lapsed capture
solution: Search this particular .avi file, find and delete it.

The Dino-Lite digital microscopes are designed to achieve the specified maximum magnification and clear focus with the scope either in contact with or on the same level as the observed object or surface, such as the skin, etc. The nozzle length is designed to provide the appropriate focal length to achieve this. When the scope is in this optimum position then the user will be able to achieve a clear focus in two distinct magnification ranges. In order to focus in the maximum magnification range simply continue to zoom beyond the first clear focus range on the observed object or surface until you see the object again come into focus. This second stage is the maximum magnification of the scope. When the scope is further away from the observed object or surface you will only be able to focus in at one magnification range.

General example how the Dino-Lite handheld digital microscopes optics works

The Dino Digital AM Series Handheld Microscopes offer magnification ranges from 10X to 220X.
The Dino Digital AM413 T5 Handheld Microscopes offer 500x fixed magnification.
The Dino Digital AM4013ZT4 Handheld Microscopes offer magnification from 400X to 470X.

Dino-eye series such as AM423 series is ~ 20X with Objective lens 40X or 100X obtain magnification 800X or 2,000X.

DinoCapture for Windows

Dino-Lite Microscopes are available with a wide variety of features and specifications with models ranging from general use to very specific applications. Some of the key distinguishing factors between different models are connectivity, resolution, magnification, working distance, and lighting.

For model number / feature key, refer to: Product Codes »

Connectivity

Most Dino-Lite microscopes connect to a Windows or Mac computer using USB. These models come with software that allows them to capture images and video, annotate, and measure(with supported models). In addition to USB models, other Dino-Lite models will connect to a TV screen via RCA/Composite Video (4012 series) or to a computer monitor using a VGA connection (4016 series).

Resolution

Dino-Lite microscopes are available in several different resolutions.

USB models:

640x480 - Basic models support up to 640x480 pixels (VGA). This is sufficient for viewing on a small to medium monitor, and capturing images for email and the web.

1280x1028 - High definition 1.3MP roughly between HDTV 720p (1MP) and 1080P (2MP). Good for viewing on medium to large monitors, capturing images for email/the web, and small to medium prints.

2592x1944 - High resolution 5MP. Good for viewing on large monitors, capturing images for email/the web, and medium to large prints.

Analog models:

510x496 (NTSC) - Composite video connection

800x600 - VGA connection

Magnification & Working Distance

Most Dino-Lite microscopes have variable magnification, ranging from about 10X to 220X. Special enhanced working distance ("L") models are available with magnifications up to 90X. Other models include the "T5" which has a fixed 500X magnification.

Lighting

Most Dino-Lite microscopes have 8 white LED lights built in. Different wavelengths and types of lighting are available for special applications.

Special lighting:

Polarized - ("ZT") models. A built-in polarizer helps reduce glare on reflective surfaces.

Strobe - ("TB") models. Strobe lighting helps capture rapidly moving objects.

Alternate wavelengths:

Near UV 390nm
Near UV-2 370nm

Infrared 850nm
Infrared-2 940nm

Blue 510nm
Green 570nm

A digital microscope is a magnifying device equipped with a digital camera. Basically, there are two types; one type is a microscope or magnifying device with a built-in digital camera while the other type is a standard style microscope adapted with a digital camera.
Dino digital microscopes function as microscope, camera, video-cam and as well as a web cam, depending on the model.

For the Dino-Lite models in the 300/3000, and 400/4000 series, the Dino-Lite consumes  1980mW (1340mW microscope +  640mW for all 8 LEDs). For the 7000 series models, the Dino-Lite consumes 2150mW (1800mW microscope + 350mW for all 8 LEDs).

Dino-Lite microscopes are WIA (Windows Image Acquisition) compatible. This means that in addition to our bundled software, the microscopes are capable of being used in a function similar to any webcam that uses WIA. Please see the following third-party link for an example of WIA being used to capture images to Microsoft Access:

http://www.ammara.com/support/samples/access-wia-camera-control.html »

All digital microscopes and eyepiece camera internal components are sealed within the housing
and require only occasional cleaning to maintain their optical integrity.

1. It is recommended that these instruments be used and stored in a clean, cool
and dry environment and that care be taken to keep them free of dust and other
contaminants.

2. Avoid exposing to fumes and extreme temperatures and never immerse or rinse in
water.

3. If cleaning is required then first attempt to gently blow off any dust or other
contaminants with compressed air. If no compressed air is available then try to
lightly brush off any lose debris with a fine brush (a makeup brush will work
well).

4. If it becomes necessary to clean the lens then we recommend using a standard
optical lens cleaning solution. To make your own optical lens cleaning solution,
mix one part alcohol (C2H5OH) with three parts ether ((C2H5)2O). Simply
saturate a cotton swab and gently wipe the lens surface clean.

5. Avoid touching the surface of the lens with your finger tips. Also it is advised to
use the plastic or other scope or eyepiece cover when not in use.

CAUTION!!! Never disassemble or open the scope or camera body. This will introduce dust
and other contaminants and may cause permanent damage.

Analog model AC adapter
Volts x Amps = Watts
Input: 100-240V, 150mA = 15 – 36 watts
Output: 5V, 800mA = 4 watts

(ESD) Electrostatic discharge -
Dino-lite Microscope Body:--- No Significant Static
Dino-lite Microscope Nozzle:--- Minor static generation*** (Internal company test test Approx 3-5K Volts)
BigCatch MS12C Portable Cradle Stand:--- No Significant Static
*** Any generated static was quickly dissipated.

(MRI) Environment Safe -
There are parts in the Dino Lite microscope as well as the USB cable that connects the Dino-Lite to a computer that may not be safe in an MRI environment.

For Temperature Rating
Internal electronic components: Temperature rating (14° F ~ 131°F) / (-10° C ~ 55° C)
External Housing (Casing) Temperature rating (14° F ~ 158°/ 176°F) / (-10° C ~ 70° C / 80°C)

For Plastic Nozzles
AD caps Models Temperature rating (14° F ~ 266°F) / (-10° C ~ 130° C)
AM front cover Models Temperature rating (14° F ~ 158°F) / (-10° C ~ 70° C)

Notes: Due to the internal adhesive, using the device in an environment over 70°C is NOT recommended. In higher temperature application it’s recommended to use a metal exterior unit.

High-pressure environment -
The Dino-Lite model AD3613 is designed with a special housing or MSAH300 Protective Enclosure that makes better suited for high pressure environments. The microscope can operate in pressures up to 125 psi if the pressure is gradually increased. The pressure should not be rapidly increased.

No, the Dino-Lite microscopes do not store any customer data in the device when the Dino-Lite digital microscope is removed and the power is turned off.

Dino-Lite handheld microscopes use EEPROM memory which are non-volatile.
The information is only setup data which is not user accessible and there is no user data path.
For more information see the "letter of volatility" »

To determine microns per pixel, measure the field of view at themagnification being used. For example, using the AD413T at its highest magnification (~220x), the FOV is 2.2mm or 2200um.

Take the FOV in microns and divide by the resolution of the Dino-Lite, so in this case we would divide 2200um by 1280 pixels. 2200um/1280 pixels = 1.72um/pixel.

Since the Dino-Lite handheld microscopes offer variable magnification, the microns per pixel will be different with each magnification. The above method can be performed for any magnification you wish.

With VGA and SVGA models, the JPEG file format is easiest to save and email. JPEG also uses least amount of storage space.

Use the BMP (AM and EM series) or TIFF (EM series only) file format for better quality image when printing or publishing.

Use TIFF, PSD or EPS formats (EM series only) if CMYK true color images are required when printing or transferring images.

RAW format

Reference of RESOULUTION VALUES

A megapixel is 1 million pixels, and is a term used not only for the number of pixels in an image, but also to express the number of image sensor elements of digital cameras or the number of display elements of digital displays.
For example, a camera with an array of 2048×1536 sensor elements is commonly said to have "3.1 megapixels" (2048 × 1536 = 3,145,728).
Therefore: Width x Height
640 x 480 = 307,200pixels / 1,000,000 = 0.3Megapixels
1280 x 1024 = 1,310,720pixels / 1,000,000 = 1.3Megapixels

VGA 640 x 480
SVGA 800 x 600
XGA 1024 x 768
WXGA 1366 x 768
SXGA 1280 x 1024
UXGA 1600 x 1200
QXGA 2048 x 1538

Pixel resolution - Under VGA Model 640 x 480 image, the resolution of a single pixel can be calculated to be
1757.3 microns/640 pixels = 2.745 microns (0.002745mm) per pixel

Pixel resolution - 1.3MP - 1280 x 1024 image, the resolution of a single pixel can be calculated to be
1757.3 microns/1280 pixels = 1.37 microns (0.00137mm) per pixel

Links for an idea of how large the different resolutions are:
640x480 - http://placehold.it/640x480 »
1280x1024 - http://placehold.it/1280x1024 »
2592x1944 - http://placehold.it/2592x1944 »

When saving images with the VGA models, you are able to achieve 300 DPI after reformatting the resolution;
For 1024 x 768 resolution can provide 2.1” x 1.6” at 300 DPI
For 1280 x 1024 resolution can provide 3.4” x 2.5” at 300 DPI

Pixel resolution - Under 640 x 480 image, the resolution of a single pixel can be calculated to be
1757.3 microns/640 pixels = 2.745 microns (0.002745mm) per pixel
Save file format allows you the option to save in 1024x768 large picture size. The JPEG format is recommended as the most common format for this but if a 100% true color match is required then you need to save in BMP or TIFF file format.

How many levels of gray could the Dino-Lite digital handheld microscopes camera see?

0.3MP image »
1.3MP image »
5MP image »
5MP example »

USB vs TV »

Metal Shell vs. ABS »

MicroTouch On/Off »

Polarizer » Examples »

The depth of field of different magnification rates has been obtained as following:
~20x --> ~3 mm (40xx series) ~ 4 mm (4xx series)
~40x --> ~1.28 mm (40xx series) ~ 1.6 mm (4xx series)
~50x --> ~0.88 mm(40xx series) ~ 1.17 mm (4xx series)
~500x--> ~ 0.09mm

Detail chart of Depth of field » (FOV and DOF)
Depth of field of AM413T, AM/D413TL »

Chart: Detail of Working Distance vs Magnification » (FOV and DOF)

The viewable area varies depending on the distance of the scope from the object. Each hand-held Dino-Lite scope has a clear protective “nozzle” which provides a “buffer” of approximately 2 cm between the scope lens and the end of the nozzle.

Distance from object viewable area
AM 4xx Series Working Distance Example
Magnification Working Distance Viewable Area
~200x -------- Zero Distance - “viewable area” ~ 0.098" (L) x 0.078" (W) / 2.5mm x 2mm
~50x -------- Zero Distance - “viewable area” ~ 0.35" (L) x 0.27" (W) / 9mm x 7mm
~40x -------- 0.8cm / 0.3” - " ~ 0.4” (L) x 0.3” (W) / 10mm x 8mm
~20x -------- 2.5 cm / 1” - " ~ 0.8” (L) x 0.6” (W) / 20mm x 15mm
~12x -------- 10.2cm / 4” - " ~ 1.5” (L) x 1.2” (W) / 40mm x 30mm

AD 4xx, AD413M(Z)T Series Working Distance Example
Magnification Working Distance
With Cap, ~50x, ~200x Touch Object.
Without Cap
Magnification Working Distance Viewable Area
~200x -------- 0.4" " ~ 0.098" (L) x 0.078" (W) / 2.5mm x 2mm
~50x -------- 0.4" " ~ 0.35" (L) x 0.27" (W) / 9mm x 7mm
~40x -------- 0.7” - " ~ 0.4” (L) x 0.3” (W) / 10mm x 8mm
~20x -------- 1.9” - " ~ 0.8” (L) x 0.6” (W) / 20mm x 15mm
~12x -------- 4.4” - " ~ 1.5” (L) x 1.2” (W) / 40mm x 30mm
*** To obtain magnifications of 60X~180X, the object must be within the clear plastic cap of the microscope which is about 20mm long.
While viewing at 60X~180X, be cautious not to touch the internal components or lens.
Here are some approximate distance to magnification of the object
placed within the clear plastic protective cap.
75x ~ 3.5mm
100x ~ 6mm
125x ~ 6mm
150x ~ 3.5mm
175x ~ 3mm
(Note that placing objects within the clear plastic cap is originally not recommended by the manufacturer.
The purpose of the cap is to protect the internals of the microscope.)

AD 4013/ 413 "L" Series (20x~92x) Working Distance Example
(Model AD413, 4013TL, AD4013ML)
Magnification Working Distance Viewable Area
~92x -------- 4.7 cm /1.9” - “viewable area” ~ 0.15” (L) x 0.11” (W) / 3.8mm x 2.85mm
~79x -------- 5.0 cm /2.0” - “viewable area” ~ 0.18” (L) x 0.13” (W) / 4.5mm x 3.3mm
~52x -------- 6.3 cm /2.5” - " ~ 0.27” (L) x 0.20” (W) / 6.8mm x 5.1mm
~27x -------- 11.5 cm /4.5” - " ~ 0.52” (L) x 0.38” (W) / 13mm x 9mm
~20x -------- 14.4 cm /5.7” - " ~ 0.7” (L) x 0.4” (W) / 18mm x 10 mm

AM4013TL-M40 (1x~37X) Estimate Magnification / Working Distance ------
Magnification -------- Working Distance --------- Viewable Area
~5X ---------------------- 16.5" / 42cm -------- 3.62" (L) x 2.25" (W) 9.5cm x 6.0cm
~10X ----------------------6.5" / 16.5cm -------- 1.75" (L) x 1.25" (W) 4.5cm x 2.9cm
~15X ----------------------4.5" / 11.5cm -------- 1.18" (L) x 0.75" (W) 3.0cm x 1.9cm
~20X ----------------------3.5" / 8.9cm -------- 0.87" (L) x 0.56" (W) 2.2cm x 1.5cm
~25X ---------------------- 2.62" / 6.6cm -------- 0.68" (L) x 0.43" (W) 1.7cm x 1.1cm
~30X ---------------------- 2.5" / 6.3cm -------- 0.56" (L) x 0.37" (W) 1.5cm x 1.0cm
~35X ---------------------- 2.25" / 5.7cm -------- 0.5" (L) x 0.31" (W) 1.2cm x 0.8cm
Detail chart of AM4013TL-M40 WD vs Magnification

AM 413MT5 / T5 (500X fixed magnification) Working Distance Example
Magnification Working Distance Viewable Area
~500x -------- 0mm~3mm “viewable area” ~ 0.026" (L) x 0.02" (W) / 0.666mm x 0.499mm

AM4013ZT4 (400X~ 480X fixed magnification) Working Distance Example
Magnification Working Distance Viewable Area
~ 480X ------------ ~ 7.5mm -------------------- 0.79mm x 0.59mm
~ 470X ------------ ~ 6.5mm --------------------0.81mm x 0.60mm
~ 460X ------------ ~ 6mm --------------------0.82mm x 0.61mm
~ 450X ------------ ~ 5mm -------------------- 0.85mm x 0.63mm
~ 440X ------------ ~ 4mm -------------------- 0.87mm x 0.65mm
~ 430X ------------ ~ 3mm -------------------- 0.88mm x 0.66mm
~ 400X~ 420X--- ~ 0-1mm --------------------0.91mmx 0.68mm

AM4013MT (Metal shell) has additional Magnification after remove len cover
Magnification / Working Distance
50x ~ 12mm
75x ~ 8.5mm
100x ~ 6mm
125x ~ 6mm
150x ~ 7.5mm
175x ~ 9mm

AM 3xxx Series Working Distance Example
Magnification Working Distance Viewable Area
~200X -------- Zero Distance - “viewable area” ~ 0.066” (L) x 0.049” (W) / 1.67mm x 1.25mm
~60x -------- Zero Distance - “viewable area” ~ 0.23” (L) x 0.17” (W) / 5.8mm x 4.3mm
~30x -------- 2cm / 0.79” - " ~ 0.4"(L) x 0.27” (W) / 10mm x 7mm
~20x -------- 5.0cm / 2.0” - " ~ 0.7” (L) / 0.4” (W) / 18mm x 10 mm
~17x -------- 10cm / 3.9” - " ~ 0.9” (L) x 0.6” (W) / 23mm x 15mm
~15x -------- 15cm / 5.9” - " ~ 1.5” (L) x 0.9” (W) / 38mm x 23mm
~10x -------- 20cm / 7.9” - " ~ 1.9” (L) x 1.5” (W) / 48mm x 38mm

AM Series with MS12C - VARIABLE VIEW RANGE CADDIE KIT
MS12C - INCLUDES BASE CADDIE PLUS TWO VARIABLE VIEW RANGE ADAPTORS
Base view range -about 0.58 inches away from object~ 40x
Base + 1 Adaptor -about 1.6 inches away from object ~ 25x
Base + 2 Adaptors-about 2.40 inches away from object ~18x magnification

Please see “Dino-Lite Relative Spectral Response” » chart

Dino-Lite microscopes operate via optical zoom. The standard USB AM4XX microscopes are optical zoom from 10X~230X. The enhanced working distance models or "L" series i.e. AD4013TL, are optical 10X~92X

All digital microscopes and eyepiece camera internal components are sealed within the housing
and require only occasional cleaning to maintain their optical integrity.

1. It is recommended that these instruments be used and stored in a clean, cool
and dry environment and that care be taken to keep them free of dust and other
contaminants.

2. Avoid exposing to fumes and extreme temperatures and never immerse or rinse in
water.

3. If cleaning is required then first attempt to gently blow off any dust or other
contaminants with compressed air. If no compressed air is available then try to
lightly brush off any lose debris with a fine brush (a makeup brush will work
well).

4. If it becomes necessary to clean the lens then we recommend using a standard
optical lens cleaning solution. To make your own optical lens cleaning solution,
mix one part alcohol (C2H5OH) with three parts ether ((C2H5)2O). Simply
saturate a cotton swab and gently wipe the lens surface clean.

5. Avoid touching the surface of the lens with your finger tips. Also it is advised to
use the plastic or other scope or eyepiece cover when not in use.

CAUTION!!! Never disassemble or open the scope or camera body. This will introduce dust
and other contaminants and may cause permanent damage.

How to do measurements with Dino-Eye
When measuring with the Dino-Eye eyepiece, type in the magnification value from the objective lens. You must calibrate first to receive proper measurements. When using the calibrated measurements, please disregard the calibrated magnification value. Below are instructions on how to calibrate the Dino-Eye.

1. In the live video window select the Calibration drop down menu (or click on the calibration button.
2. Select “New Calibration Profile”.

3. A small window will pop up. Give the profile a name.

4. Click “Continue Calibration” when done naming the profile.
5. In the new menu. Press “F8” or “Freeze” button to freeze the calibration object.

6. Enter the magnification value of the objective lens. Press Enter when finished.
7. A red bar with two blue dotes will appear with pink guidance lines, you are now ready to calibrate.

8. Click on one of the blue dots to start moving the blue dot to your desired location.
9. Click again to stop.
10. Click on the other blue dot to start setting the other end point.
11. Click on the left mouse button again to stop.
12. When the correct distance is measured, enter the known distance.
13. Enter the unit of measure by selecting the “Unit” dropdown box.

14. Press “Finish” when done.
15. Now the Dino-Eye is calibrated to your microscope.

Below are several example configurations, along with an estimate of the human eye field of view (FOV) for comparison.

Compound Microscope

The AM series eyepiece cameras are equivalent to a 20x eyepiece (most traditional eyepiece are using 16x or 10x). Thus for a given objective lens, the eyepiece cameras will usually provide a smaller field of view and larger magnification of the original image when compared to the traditional eyepiece. To achieve a larger field of view with the DinoEye, use a less powerful objective lens, or attach a reducer lens such as a 0.5x lens.

There are two common sized apertures on a microscope: 23mm and 30mm. If you choose
AM4023 is perfect for 23mm Aperture.

If the inner diameter of your eyepiece slot is 30mm or 30.5mm then the AM4023X is another
versatile digital eyepiece. A unique feature it has is that it is capable of being adjusted for focus
because of its tube design that has a 23mm aperture from top to bottom. Included with the
AM4023X are two adapters which are 30mm aperture and 30.5mm aperture.

If you have a unique aperture where other eyepieces would not fit. The AM4023U would be
useful and may able to meet your requirement The way the AM4023U works is that it is made to
be placed over an existing eyepiece that has a 36mm outer diameter or smaller. The AM4023U is
made to see through your current existing eyepiece which eliminates the need to figure out your
microscopes eyepiece slot aperture.

The AM4023 series eyepiece camera is designed with a zoom-in feature. The field of view
(FOV) is approx. 12-14mm allowing for a high quality image of a reduced area.

With a near UV light digital Camera you can capture very detailed data, which can be undetected by human eyes. Below are images depicting the greater detail (scratches, blemished, etc.)
that can be captured when using a UV camera versus a standard camera.
SURFACE INSPECTION
IDENTIFICATION
3D INSPECTION SELECTION
PCB INSPECTION/SOLDER INSPECTION

What Is Ultraviolet Light?
Ultraviolet light is part of the light spectrum, which is classified into three wavelength ranges:
UV-C from 100 nanometers to 280 nm;
UV-B from 280nm to 315 nm; and,
UV-A from 315 nm to 400nm.
These waves are invisible to the human eye, but some insects like bumblebees, can see them. The human eye responds to light with wavelengths from about 400 nm (violet) to 700 nm (red).

Visible light waves are the only electromagnetic waves we can see. We see these waves as the colors of the rainbow. Each color has a different wavelength. Red has the longest wavelength and violet has the shortest. When all the waves are seen together, they make white light.

What Is the Dino-Lite near UV series light output power (mW or mW/cm2)?
The light output power of the near UV LED for
AD4013T-FVW is about 16mW x 4 LED = 64mW
AM4013-FVT is about 16mW x 8 LED = 128mW
AM4013T-FV2W is about 11mW x 4 LED = 44mW

The diverge angle for AM-413FVT2-- 40o

The Dino-Lite can see 256 levels of gray.

Dino-Lite scope lens are glass with IR coated. The filter is an IR pass filter.
The Dino-Lite digital microscopes are sensitive up to 940nm
NOTE: Only the AM413FIT (850nm) has IR cut filter, and AD413T-I2V (IR 940nm) has no IR cut filter.
That is the center value of the wavelength. Since it is LED, not laser, the wavelength has bandwidth.
The AM413-FIT (IR) and AM4113FVT (UV) contain visible light filters. Other models with variable lighting, like the AD413T-I2V (UV/IR) do not contain visible light filters. These models are most effective in imaging UV and IR at distances of less than 3 inches..

for near UV
• Excitation: near-UV LEDs (on/off software adjustable), 390-400nm
• Excitation: near-UV LEDs 365nm~375nm
• Emission Filter Cutoff Wavelength: 425nm
The light output power of the UV LED for AM4013FVW is about 16mW x 8 LED = 64mW
The light output power of the UV LED for AM4013T-FV2W is about 11mW x 4 LED = 44mW
Dino-Lite (UV and IR) contain filters for visible light?
Some models, including the AM413-FIT (IR) and AM4013FVT (UV) contain visible light filters. Other models with variable lighting, like the AD413T-I2V (UV/IR) do not contain visible light filters. These models are most effective in imaging UV and IR at distances of less than 3 inches.

Raw image files are unprocessed and uncompressed image data files.To convert your processed BMP into a raw file format for the purposes of compatibility, you will need to use third party software available online. One such software is available here:

http://www.softpedia.com/get/Multimedia/Graphic/Image-Convertors/Batch-Image-Converter-LLS.shtml »

About DinoCapture 1.0 / 2.0 measurement feature.
The Dino-Lite Pro microscope has higher measurement accuracy at higher magnifications such as 50x or 200x. Generally you can expect up to about 3% deviation from the correct measurement results. Software features such as the picture-in-picture magnifier or automatic edge detection can lower the deviation to 2% or less.
You can calibrate the software to acquire more accurate measurements. (Only with the measurement models of the 4xx/4xxx/7xxx series).

About Dino-Capture Accuracy of Measurement

Calibration averaging procedure in DinoCapture 2.0
By making several calibration profiles at different magnification levels, the software can average the calibrated data to better improve the accuracy of measurements taken within the calibrated magnification range We recommend calibrating at a minimum of 3 magnifications level.

About Dino-Capture 2.0 Calibration
How to calibrate the dino-lite microscope

Limitations of the measurement feature:
-The measurements only can measure two dimensions (X,Y).

The term Mil means one thousandth of an inch (0.001 inch).
A circular mil is the area of a circular wire with a diameter
of one mil, 0.001". ...
Current we can select mm = 1,000um (micrometer / microns) = 0.1 cm

mil = 0.001 inch.
inch= 1,000 mil.
um = 0.001 mm
mm = 1,000 um (micrometer / microns)

Video Demo - Video Library »

Auto Distance Measurement Quick Guide»

Measurement with Dino-Eye Eyepiece

1. In the live video window select the Calibration drop down menu (or click on the calibration button.
2. Select “New Calibration Profile”.

3. A small window will pop up. Give the profile a name.

4. Click “Continue Calibration” when done naming the profile.
5. In the new menu. Press “F8” or “Freeze” button to freeze the calibration object.

6. Enter the magnification value of the objective lens. Press Enter when finished.
7. A red bar with two blue dotes will appear with pink guidance lines, you are now ready to calibrate.

8. Click on one of the blue dots to start moving the blue dot to your desired location.
9. Click again to stop.
10. Click on the other blue dot to start setting the other end point.
11. Click on the left mouse button again to stop.
12. When the correct distance is measured, enter the known distance.
13. Enter the unit of measure by selecting the “Unit” dropdown box.

14. Press “Finish” when done.
15. Now the Dino-Eye is calibrated to your microscope.

The 5 megapixel (2592 x 1944 pixel) resolution Dino-Lite models require the third-party MJPEG codec to be installed in order to display at the maximum resolution. The 5 megapixel resolution is not currently supported on Mac OS.

On Windows, DinoCapture will check for the MJPEG codec and alert the user if it is missing. Many computers come with this codec already installed, in which case no additional software is necessary. Different versions of the MJPEG codec may also have different performance depending on the exact specifications of the computer in use. For these reasons, the Dino-Lite does not include the MJPEG codec.

For those computers that do not already have the codec installed, several freeware and trial versions are available online. Since different versions may perform differently depending on your computers specifications, we might suggest downloading a trial version first to confirm the performance is satisfactory. Several websites offering free or paid versions are listed below:

http://www.free-codecs.com/download/motion_jpeg_codec.htm (free limited version or $25 full version)

http://www.morgan-multimedia.com/technicalsV3.htm (60 day trial version or $20 full version)

About the Dino-Lite SDK

An available free SDK (software development kit) provides C++, C#, and Visual Basic code and OCX component files for custom applications once a software license agreement is signed. The SDK includes documentation in the form of a PDF file which can be found in the base install directory. (Typically: C:Program files (x86)/DNVideoX SDK/)

How to access the SDK?

Please download and complete the form below and submit it with a brief description of your application to [email protected]» or by fax to 310-618-9996. After we receive the required information we will respond with a link to download the SDK within 1 to 2 business days.

SDK License Agreement»

Scientific/Academic
Matlab: Compatible (Matlab Image Acquisition Toolbox software)
LabView: Appears to be compatible (We have also had customers report success using the microscope with LabView using an add-on called CamViewIT. You can find more information about CamViewIT here - http://www.inventeering.com/products-CamViewIT_Toolkit_for_LabVIEW.htm)

Imaging
AMCAP: Compatible
Minisee: Compatible

Conferencing/Chat
Skype: Compatible
MSN: Compatible
Google chat: Compatible

Jewelry
Edge Jewelry Software: Appears to be compatible (may need to contact Edge support for initial set up)

Download the 3.5" LCD Display firmware update from the following link
For S/N-9604xx, 9Bxxxx, 02xxxx Firmware for 3.5" LCD Display
http://bigc.com/download/AMK4X-CMF401-20091211.rar »

For S/N-70xxxx-95xxxx Firmware for 3.5" LCD Display
http://bigc.com/download/AMK412_12_25-20081225.rar »

Unzip the file onto your SD Memory Card (support up to 16G class6 SD card). Insert the SD Memory Card into the AMK412-C200 / AMK4012-C200, make sure your AMK4xxx-C200 is plugged into the AC Adaptor before upgrading Turn on your AMK4xxxx-C200.
Press the Menu button 3 times. (This should cycle to Picture, Video, then finally Menu)
Scroll down on the menu and select Firmware Update

More than one microscope can be operated simultaneously using the DinoCapture 2.0 software which is available for download here:
http://bigc.com/downloads/»

The ability to run multiple microscopes simultaneously can be quite resource intensive and thus the number that will work with a given computer depends on the specs of that computer.
For reference, the table below shows approximately how many simultaneous microscopes should run on a given computer:
System: number of simultaneous Dino-Lite microscopes
Intel Core 2: 2
Intel i3/i5: 3
Intel i7: 4

If you are having trouble running multiple cameras simultaneously, please check the following:
All microscopes are connected to a USB 2.0 port (not USB 1.1)
Microscopes are connected directly to the computer (not through a hub or extension cable)
If using a laptop, power source is connected.
No other resource intensive USB devices or webcams are currently operating.
Try lowering the resolution of the Dino-Lite microscopes to 640x480.

The DinoCapture (Windows) or DinoXcope (Mac OS) software is included on the CD that came with the Dino-Lite. It is also available for download from our website. If you have an older version, the software may update automatically (if you have not disabled that feature), or you can update manually by downloading the latest version. To install by downloading the latest version from this website, follow the instructions below:

Windows Install

1. Download DinoCapture or Insert CD and run Setup	2. Click yes if prompted
3. Click next and install	4. Double click DinoCapture icon

Once you have purchased a Dino-Lite digital microscope you are free to use the DinoCapture software on as many computers as needed at no extra cost. As you are installing the DinoCapture software the license agreement must be agreed with, in order to complete this installation. There you will find detailed information about the license statement. Any and all optional software updates are free of cost.

http://bigc.com/download/pdf/IP Camera Setup _20110512.pdf »

http://bigc.com/download/pdf/Auto Distance Measurement Quick Guide_20110512.pdf »

DinoCapture 2.0

http://bigc.com/download/Manual/Manual_dnc2_10%20PDF.pdf »

IP Camera Mode

IP Camera Setup»

Unfortunately the model AM211 is not compatible with current Mac OS editions, although it will still work on older versions (Mac OS 10.4 and below). This model is the very first Dino-Lite and was discontinued several years ago. It has been replaced with model AM2011 which is compatible with Mac OS 10.5 and above. For more information about that model, please see the product page: AM2011 »

The DinoCapture (Windows) or DinoXcope (Mac OS) software is included on the CD that came with the
Dino-Lite. It is also available for download from our website. If you have an older version, the software
may update automatically (if you have not disabled that feature), or you can update manually by
downloading the latest version. To install by downloading the latest version from this website, follow
the instructions below

Mac OS Install

1. Download DinoXcope or Insert CD	2. Double click install package
3. Click Install	4. Double click DinoXcope icon

If you are currently using DinoScope software, please go to our Downloads section and download DinoXcope*
you can also download by clicking this link:

http://www.bigc.com/downloads/drivers-and-software/ »

*DinoXcope only for users with MAC OSX 10.4 or later with AM2011, AM3XX~AM4XX DinoLite Microscopes.

Please uninstall DinoScope before installing DinoXcope.
DinoXcope will not work if DinoScope is uninstalled after the DinoXcope installation. Alternatively you may choose to leave DinoScope on your system at the same time as DinoXcope,
Please note that uninstalling either program will cause the other to not work from that point on.

There are two ways you can save images as .JPEG or .PNG format in DinoXcope.
1. Double click on the already captured picture thumbnail on the left side of the window. Click on the "Save As" button in the measurement window. Select the desired image format.

2. Right-click or control key + click, then select: "Save As" from the contextual popup menu. Both methods will bring up a "Save As" dialog and at the bottom of the dialog the file format can be selected from the format popup menu.

There are 2 ways to delete an image from DinoXcope.
1. You can click on the image you wish to delete and press the Delete button on the keyboard.
2. You can click on the image you wish to delete and drag it to the Trash.

If you wish to delete multiple images from DinoXcope:
Select the image you wish to delete by clicking on the thumbnail, select more than one image by holding down on the "Apple/Command" key and selecting other images then press the Delete button on the keyboard.
(Selecting images using the "Shift" key will select the full range of images from first click to second click.)

Press Command+Shift+S in full screen mode to capture images.

To adjust image settings, from the top menu in DinoXcope click on Controls then select Video Settings.

DinoXcope saves videos in QuickTime (.mov) file format

To find the instructions for DinoXcope, go to the Help section while in the DinoXcope software. You can either search for the specific issue or click on DinoXcope Help. Or go to the download section on our website and download the PDF file - "DinoXcope user guide".

DinoXcope is for use with MAC OS 10.4 or later.